![]() ![]() ![]() The highest mean numbers of shoots and well-developed roots were obtained on LS medium containing solely 0.5 mg/L TDZ, producing 5.3 shoots and 3.3 roots per explant after 2 weeks of incubation. To establish an efficient regeneration system using nodal segments excised from 4-week-old germinated seedlings, direct plant regeneration, without additional rooting stage, was achieved on LS medium containing 0.5 mg/L 6-benzylaminopurine (BAP), kinetin (KIN), thidiazuron (TDZ), or gibberellic acid (GA3), alone or in combination with 0.25 mg/L indole-3-acetic acid (IAA) or indole-3- butyric acid (IBA), after 2 weeks of incubation. contains polyphenols and carotenoids with antiinflammatory and antioxidant activities used against diabetes. Keywords: Cape gooseberry, withanolides, squalene synthase, HPLC, qPCR1. The relative expression level of squalene synthase gene showed consistent results with the changes in withanolide levels that 1.80- and 10.13-fold increase for 2- and 5-hours mild high-temperature exposure times, and 1.34- and 2.01-fold increase in 15 min and 3 hours UV-B exposure times, respectively. Exposure to UV-B also changed the withanolide content by 7.22- and 7-fold increase for 15 min and 3 hours exposure times, respectively. We demonstrated significant changes in withanolide content with 7.86- and 12.5-fold increase for 2- and 5-hours mild high-temperature exposure times, respectively. peruviana shoot cultures exposed to mild non-lethal heat stress (45 ☌ for 2 and 5 hours) and UV-B radiation (313 nm for 15 min and 3 hours). In this study, we investigated changes in withanolide accumulation and characterized the gene expression level changes of squalene synthase enzyme in P. Exogenous addition of elicitors is considered as the most effective strategy for enhanced production of secondary metabolites. ![]() To meet the increasing demands for these compounds, plant cell technology offers a reliable alternative. These withanolides are high-value drug candidates due to their various pharmacological properties. (Cape gooseberry) is a source for a variety of phytocompounds suchĪs withanolides, withanone, withaferin A and withanolide A. ![]()
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